Tài liệu Nuôi cấy tế bào huyền phù của cây nghệ đen (Curcuma zedoaria Roscoe)

Introduction
Zedoary (Curcuma zedoaria Roscoe) plant, a vegetatively propagated species of the Zingiberaceae family, is an aromatic herbaceous plant with a rhizome growing mainly in South Asian and South-East Asian countries, and China [1]. Zedoary is a valuable
medicinal plant, the essential oil obtained from rhizome has been reported to have antimicrobial activity and be clinically used in the treatment of cervical cancer, the water extract of Zedoary demonstrated antimutagenic activity [2]. It has been also used for stomach diseases, hepatoprotection [3], treatment of blood stagnation, and promoting menstruation as a traditional medicine in Asia [4]. Furthermore, the Zedoary has anti-inflammatory potency related to its antioxidant effects [3]. Higher plants are a valuable source of wide range of secondary metabolites, which are used as pharmaceuticals, agrochemicals, flavours, fragrances, colours, biopesticides and food
additives [5]. In the end of 1960s, plant cell culture technologies were introduced as a tool for both studying and producing plant secondary metabolites. A highly potent secondary metabolites that is used in pharmaceuticals and food additives have been
produced through plant cell suspension cultures in large-scale [6, 7]. Cell suspension culture is a requirement for the production of chemicals from plants in a way quite similar to that used for microorganisms, where the utilization of bioreactor becomes feasible [8]. The purpose of this study is to establish an efficient suspension cell culture protocol for C. zedoaria as a starting point to produce bioactive compounds in plant cell culture.



TÓM TẮT (ABSTRACT)

Nghiên cứu này trình bày phương thức nuôi cấy callus và tế bào huyền phù của cây nghệ đen
(Curcuma zedoaria Roscoe). Môi trường MS bổ sung 2% sucrose; 1,0 mg/l 2,4-D và 1,0 mg/l BA
thích hợp cho nuôi cấy callus từ bẹ lá của cây nghệ đen in vitro. Trong quá trình nuôi cấy, các callus
thứ cấp được tạo thành từ callus sơ cấp trên môi trường MS có bổ sung 0,5 mg/l 2.4-D và 0,5 mg/l
BA. Các callus này có màu vàng, rắn và rời rạc. Nuôi cấy tế bào huyền phù được thiết lập với 3 g sinh
khối callus tươi nuôi trong bình tam giác thể tích 250 ml, chứa 50 ml môi trường MS có bổ sung 3%
sucrose; 1,5 mg/l 2,4-D và 0,5 mg/l BA với tốc độ lắc 120 vòng/phút. Sinh khối cao nhất đạt 10,44 g
trọng lượng tươi (0,66 g trọng lượng khô) sau 14 ngày nuôi cấy. Các kết quả này là cơ sở cho những
nghiên cứu sâu hơn về cây nghệ đen nhằm cung cấp nguồn nguyên liệu tế bào để sản xuất các hợp chất
thứ cấp ở qui mô lớn.

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