Sách Cellular and Molecular in Neroscience research

CONTENTS


Foreword vii

Preface ix

Contributors . xiii

1 Analyses of Intracellular Signal Transduction Pathways in CNS

Progenitor Cells 1

Elena Cattaneo and Luciano Conti

2 Confocal and Electron Microscopic Tracking of Internalized

Neuropeptides and/or Their Receptors 15

Alain Beaudet, Alexander C. Jackson, and Franck Vandenbulcke

3 Transfection Methods for Neurons in Primary Culture 29

Christoph Kaether, Martin Köhrmann, Carlos G. Dotti,

and Francesca Ruberti

4 Polyethylenimine: a Versatile Cationic Polymer for Plasmid-Based Gene

Delivery in the CNS .37

Barbara A. Demeneix, Gregory F. Lemkine, and Hajer Guissouma

5 Transfection of GABAA Receptor with GFP-Tagged Subunits

in Neurons and HEK 293 Cells 53

Stefano Vicini, Jin Hong Li, Wei Jian Zhu, Karl Krueger,

and Jian Feng Wang

6 Neuronal Transfection Using Particle-Mediated Gene Transfer 67

Harold Gainer, Raymond L. Fields, and Shirley B. House

7 Analysis of Gene Expression in Genetically Labeled Single Cells . 85

Stefano Gustincich, Andreas Feigenspan, and Elio Raviola

8 Immunocytochemistry and In Situ Hybridization:

Their Combinations for Cytofunctional Approaches

of Central and Peripheral Neurons . 119

Marc Landry and André Calas

9 In Situ Reverse Transcription PCR for Detection of mRNA

in the CNS 145

Helle Broholm and Steen Gammeltoft

10 Immunocytochemical Labeling Methods and Related Techniques

for Ultrastructural Analysis of Neuronal Connectivity . 161

Patrizia Aimar, Laura Lossi, and Adalberto Merighi

11 Combined Electrophysiological and Morphological Analyses of

CNS Neurons 181

Alfredo Ribeiro-da-Silva and Yves De Koninck

12 Tract Tracing Methods at the Light Microscopic Level . 203

Marina Bentivoglio and Giuseppe Bertini

13 Tract Tracing Methods at the Ultrastructural Level . 221

Isaura Tavares, Armando Almeida, and Deolinda Lima

14 In Vivo Analysis of Cell Proliferation and Apoptosis in the CNS . 235

Laura Lossi, Silvia Mioletti, Patrizia Aimar, Renato Bruno,

and Adalberto Merighi

15 Confocal Imaging of Nerve Cells and Their Connections 259

Andrew J. Todd

16 Confocal Imaging of Calcium Signaling in Cells

from Acute Brain Slices . . 273

Wim Scheenen and Giorgio Carmignoto

Index . . 285