Tài liệu BIOPHYSICS - Edited by A.N. Misra

BIOPHYSICS
Edited by A.N. Misra

ContentsPreface IX
Chapter 1 Laser Correlation Spectroscopy:
Nutritional, Ecological and Toxic Aspects 1
M. Karganov, I. Alchinova, E. Arkhipova and A.V. Skalny
Chapter 2 “Flow and Rate”: Concept and Clinical
Applications of a New Hemodynamic Theory 17
Sayed Nour
Chapter 3 Microtools for Microsurgery of a
Single Cell in Field of Cellular Engineering 77
Vladimir A. Nikitin
Chapter 4 An Interfacial Thermodynamics
Model for Protein Stability 91
Donald J. Jacobs
Chapter 5 Recent Developments in the Study of
the Behavior of Fluorescent Membrane
Probes in Lipid Bilayers: Molecular
Dynamics Approach 133
Luís M.S. Loura, A.J. Palace Carvalho
and J.P. Prates Ramalho
Chapter 6 Thermoluminescence in Chloroplast Thylakoid 155
Amarendra Narayan Misra,
Meena Misra and Ranjeet Singh
Chapter 7 Chlorophyll Fluorescence in Plant Biology 171
Amarendra Narayan Misra,
Meena Misra and Ranjeet Singh
Chapter 8 Electromagnetic Radiation and Life:
Bioelementological Point of View 193
A.Kh. Tambiev and A.V. Skalny


Dedicated to my parents Dr. Sachidananda Misra and Mrs. Sailabala Misra.


Preface
Biophysics is a vast cross-disciplinary subject encompassing the fields of biology,
physics and computational biology etc in microbes, plants, animals and human
being. Wide array of subjects from molecular, physiological and structural are
covered in this book. Most of these chapters are oriented toward new techniques or
the application of techniques in the novel fields. The contributions from scientists
and experts from different continents and countries focuss on major aspects of
biophysics. The book covers a wide range of topics reflecting the complexity of the
biological systems.
Although the field of biophysics is ever emerging and innovative, the recent topics
covered in this book are contemporary and application-oriented in the field of
biology, agriculture, and medicine. This book contains mainly reviews of
photobiology, molecular motors, medical biophysics such as micotools and
hoemodynamic theory.
Topics on Photobiology deal with Electromagnetic radiation and life: bioelementological
point of view; Laser correlation spectroscopy approach to bioelementology problems:
nutritional, ecological and toxic aspects; Chlorophyll fluorescence in plant biology,
Thermoluminescence in chloroplast thylakoid. Topics on biotechnology and medical
biophysics deal with molecular motors, micotools and application of hoemodynamic
theory in mechanical cardiac assist devices.
Sun is the main source of electromagnetic radiation on earth that sustains life that
has practical application in various fields: medicine, biology, agriculture and
biotechnology.
The organization and structure/function relationships of biomembranes area represent
substantial number of biochemical and biophysics probes. Fluorescence techniques are
frequently used as a tools to characterize the structure and dynamics of
biomembranes. However, a novel computer simulation method for the study of
molecular dynamics (MD) of membranes for atomic-scale information using
fluorescent membrane probes is described. The chapters on chlorophyll fluorescence
X Preface
in plant biology and thermoluminescence in chloroplast thylakoid deal with the
aspects of theory, technique and applications in biomembranes.
Bioelements are building blocks of life and physical-chemical principles regulate all
the elemental behavior for the life processes to continue in the living system.
Interesting introductory chapter describes how this phenomenon ios regulated by light
and explains the method of laser correlation spectroscopy that measures this
phenomenon in a living system.
Polyhydroxyalkanoates (PHAs) is biopolymer produced by various microbes. In this
book the authors have projected luminous bacteria as a novel potential PHA producer.
That can be greatly used in biotechnology.
Energetics of biomolecular machines or molecular motors, is calculated in the chapter
for Molecular Motors by calculating flux and free energy. This is used for predicting
the conformational transitions and the extent to which the power stroke and the
thermal ratchet mechanisms that take part in the molecular motor motion.
Nowdays microtools are used in microsurgery of single cells and microscopic things
such as cells or organelles. Microsurgery of a single cell, replacing its elements, the
introduction of foreign genetic material makes this area of medicine, agriculture, basic
and experimental biology especially relevant. Appearance in the hands of
microsurgeon of a single cell of the new microtools that have the ability of active
interference in functioning of the cell without causing a significant damage to it have
changed the experimental biology.
Cardiovascular diseases (CVD) still cause the highest mortality in developed
countries. Recently, congestive heart failure (CHF) was defined by the NIH as the new
epidemic in USA with 5 millions new cases per year. Mechanical cardiac assist devices
(CAD) are currently indicated for CHF patients. “Flow and Rate”: Concept and clinical
applications of a new hemodynamic theory deals with transplantation problems and
how to overcome them.
I would like to express my sincere thanks to the authors around the world for their
excellent contributions to the book. I hope that the book will enhance the knowledge
and give an insight into the present problems in biophysics. I express my appreciation
to all the authors of this book.
Last but not the least, I express sincere thanks to my daughter Eva, son Rishi, sonin-
law Divya and my granddaughter Diksha for their love, affection,
encouragements and assistance during the preparation of this book which gave me
immense inspiration for devoting my time and energy to this work. My parents,
brothers and sisters and their family members have been a constant source of
encouragement for my work and endeavours. I am indebted to them. There is none
but my teachers at all levels who contributed to my knowledge and have been a
perpetual source of aspiration for all constructive academic work, that I have ever
done in any sphere of my life. I owe them this piece of work and dedicate this book
to them.
The assistance of InTech team is admirable and is willfully acknowledged.
Prof. Dr. A.N. Misra
Post-Graduate Department of Biosciences & Biotechnology
Fakir Mohan University, Balasore &
Centre for Life Sciences, School of Natural Sciences
Central University of Jharkhand, Ratu-Lohardaga Road, Brambe, Ranchi
India